Journal: Molecules

Article Title: Bifunctional Bioactive Polymer Surfaces with Micrometer and Submicrometer-Sized Structure: The Effects of Structure Spacing and Elastic Modulus on Bioactivity

doi: 10.3390/molecules24183371

Figure Lengend Snippet: Normalized relative dye reduction (in %) due to keratinocytes grown on the structured and functionalized samples: ( a ). SMAMP@Au_Si with 200 nm to 2 µm spacing; ( b ). SMAMP@Au_PSB@Si with 200 nm to 2 µm spacing; ( c ). SMAMP@PSB samples with 1 µm to 8.5 µm spacing; ( d ). SMAMP monolayer and PSB monolayer , and SMAMP network and PSB network . The data for each sample was recorded after 24, 48, and 72 h of incubation time, respectively. The error bars are the standard deviation calculated from two independent experiments using three replicates each.

Article Snippet: As previously reported [ , ], gingival mucosal keratinocytes (GM-K), immortalized with HPV-16, were cultured in keratinocytes growth medium (KGM, Promocell, Heidelberg, Germany) with accompanying supplement at concentrations as supplied by the manufacturer: bovine pituitary extract: 0.004 mg mL −1 ; insulin: 5 μg mL −1 ; epidermal growth factor (EGF): 0.125 ng mL −1 ; hydrocortisone: 0.33 µg mL −1 ; epinephrine: 0.39 µg mL −1 ; transferrin: 10 μg mL −1 ; kanamycin: 100 μg mL −1 ; CaCl 2 : 0.06 mM.

Techniques: Incubation, Standard Deviation